The inhibitory effect of α-methyl-5-HT on ATP-activated currents in rat dorsal root ganglion neurons

Abstract The purpose of this study was to explore the inhibitory effect α-methyl-5- hydroxytryptamine (α-methyl-5-HT) on ATP-activated current (IATP) in rat dorsal root ganglion (DRG) neurons. Whole-cell patch clamp experiment performed cultured DRG One minute after treatment with α-methyl-5-HT, ATP (10-4 mol/L) activation neurons inhibited. However, independent caused by α-methyl-5-HT. dose-response curve for IATP showed that α-methyl-5-HT significantly shifted it. Kd values currents before and pre-addition were similar (4.23×10-5 mol/L vs. 6.81×10-5 mol/L). Furthermore, cyproheptadine (10-6 mol/L), an antagonist 5-HT2 receptor, can reverse inhibition After intracellular dialysis KN93 (CaMKII inhibitor) H7 (PKC inhibitor), also completely eliminated. In conclusion, our results inhibited through activating receptor resulting phosphorylation receptor. It G protein coupled corresponding signaling transduction cascade.